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LASCPC-01
LASCPC-01
規格:
貨期:
編號:B161409
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 LASCPC-01
商品貨號 B161409
Organism Homo sapiens, human
Tissue prostate
Product Format frozen 1.0 mL
Morphology aggregate
Culture Properties suspension/aggregate
Biosafety Level 2  [Cells contain lentivirus genes]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease prostate cancer
Age 75-80
Gender male
Ethnicity Caucasian
Applications This cell line with the N-Myc as a driver of NEPC can serve for studying therapeutic treatments of Neuroendocrine prostate cancer (NEPC)
Karyotype Conventional karyotyping: normal 46 X,Y
Array CGH: mosaic gian of 1q23.1-1q44
Images Cell Micrograph of LASCPC-01 cells, ATCC CRL-3356
Clinical Data 75-80 years
male
Caucasian
Comments Expression of RFP, GFP, MYCN, and myristoylated AKT1.
Complete Growth Medium

To prepare 500mL of complete medium:

  • 464 mL Modified HITES medium (prepared as below)
  • 1 mL Hydrocortisone (Sigma cat# H-0135; 10 nM or 1.0 x 10-8 M)
  • 5 mL Transferrin (Sigma cat# T-5391; 0.01 mg/mL or 10 µg/mL)
  • 25 mL FBS (ATCC 30-2020)
  • 5 mL L-Glutamine (ATCC 30-2214)
Modified HITES Medium
  • Insulin, human recombinant (Life Technologies cat# 12585-014), 50 mg 
  • Sodium Selenite (Sigma cat# S9133), 0.04326 mg 
  • Β-Estradiol (Sigma cat# E2257), 0.02724 mg 
  • L-Glutamine (ATCC 30-2214), 2.922 g 
  • RPMI-1640 (ATCC 30-2001), 10 L
Adjust pH to 7.2 – 7.4. Filter sterilize through a 0.2 micrometer filter, dispense 500 mL into 500 mL bottles.

Note: Complete culture medium is stable for 1 month once hydrocortisone and transferrin are added.
Subculturing Cultures can be maintained by addition of fresh medium once or twice a week.
Cryopreservation liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Cells per Vial Since being aggregates we use 20 ml culture medium with healthy aggregates per 1 ml freeze volume
Volume 1.0 mL
STR Profile
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 12
D16S539: 11
D5S818: 11
D7S820: 8,10
THO1: 9
TPOX: 8,11
vWA: 15,17
Sterility Tests Bacteria and yeast: No growth 
Mycoplasma: No growth
Viability ≥ 50%
Population Doubling Time Approximately 24 hours per depositor
Name of Depositor J Lee
Year of Origin 2014
References

Lee JK, et al. N-Myc drives neuroendocrine prostate cancer initiated from human prostate epithelial cells. Canc Cell 29(4):536-47, 2016. PubMed: 27050099

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