国产欧美另类久久精品蜜芽-色播久久人人爽人人爽人人片av-久久发布国产伦子伦精品-又黄又硬又湿又刺激视频免费-女人喷液抽搐高潮视频

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > MyC-CaP
▍最近瀏覽歷史
▍聯系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮海區莊市街道興莊路9號
  • 創e慧谷42號樓B幢401室
MyC-CaP
MyC-CaP
規格:
貨期:
編號:B161410
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 MyC-CaP
商品貨號 B161410
Organism Mus musculus, mouse
Tissue prostate
Product Format frozen
Morphology epithelial-like
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease prostate cancer
Age 16 months
Gender male
Strain FVB; Hi-Myc transgenic
Applications Study of prostate cancer progression from androgen dependence to a hormone-refractory or androgen-independent stage
Storage Conditions liquid nitrogen vapor phase
Images Cell Micrograph of MyC-CaP Mouse Prostate Cells
Derivation The MyC-CaP cell line was derived from a genetically engineered mouse prostate cancer removed from an animal that was never exposed to hormone ablation.
Receptor Expression wild-type androgen receptor (AR)
Comments The cells have an amplified androgen receptor gene despite no prior exposure to androgen withdrawal. These cells express wild-type androgen receptor (AR), retain androgen-dependent transgene expression and demonstrate androgen-dependent growth on soft agar and in mice.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Seeding Density 5 x103 to 1 x 104 viable cells/cm2
Subculturing Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) (ATCC® 30-2200) or 0.05% Trypsin – 0.02% EDTA for Primary Cells (ATCC® PCS-999-003) solution to remove all traces of serum which contains trypsin inhibitor.
  2. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  3. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  4. Transfer cell suspension to a centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes.
  5. Discard supernatant. Resuspend the cell pellet in fresh growth medium.
  6. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.

Subcultivation ratio: 1:5 to 1:20 is recommended.

Medium renewal: 2 to 3 times a week
Cryopreservation complete growth medium, 90%; DMSO, 10%
Culture Conditions Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Population Doubling Time 18 hours
Name of Depositor CL Sawyers
Year of Origin 2005
References

Watson PA, et al. Context-dependent hormone-refractory progression revealed through characterization of a novel murine prostate cancer cell line. Cancer Res. 65(24): 11565-11571, 2005. PubMed: 16357166

梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479