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BMEC-1
BMEC-1
規(guī)格:
貨期:
編號:B161699
品牌:Mingzhoubio

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產(chǎn)品名稱 BMEC-1
商品貨號 B161699
Organism Homo sapiens, human
Tissue bone marrow
Product Format frozen 1.0 mL
Morphology epithelial-like
Culture Properties adherent
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications Studies in Cancer, tumor metastasis, endothelial function, cell trafficking, surface molecule interactions, etc.
Storage Conditions liquid nitrogen vapor phase
Images
Comments This cell line was infected with SV-40 Large T antigen. The depositor stated that the cell line has passage limitation ≤ 27. Therefore, harvest prior to passage 27.
Complete Growth Medium

The base medium for this cell line is MCDB-131 (Gibco cat # 10372-019). To make the complete medium add to 500 mL of the base medium:

  • 56 mL Fetal Bovine Serum (FBS; ATCC 30-2020)
Subculturing
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with DPBS (ATCC catalog # 30-2200) to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
    Cultures can be established between 2.0 x 104 and 4.0 x 104 viable cells/cm2.
  6. Incubate cultures at 37°C.
Interval: Maintain cultures at a cell concentration between 1.0 x 104 and 3.0 x 104 cell/cm2.
Subcultivation Ratio: A subcultivation ratio of 1:8 to 1:10 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation Complete growth media 95% + 5% DMSO (ATCC 4-X)
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5% CO2
Temperature: 37°C
Cells per Vial Approximately 2.0 to 3.0 x 106 cells
Volume 1.0 mL
STR Profile
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 12
D16S539: 9,10
D5S818: 12
D7S820: 8,10
THO1: 7,9.3
TPOX: 8,9
vWA: 14,17
Sterility Tests Bacteria and yeast: No growth
Mycoplasma: No growth
Viability ≥ 50%
Name of Depositor J Moon, National Center for Emerging and Zoonotic Infectious Diseases, CDC
Year of Origin 1995
References

Candal FJ, et al. BMEC-1: a human bone marrow microvascular endothelial cell line with primary cell characteristics. Microvasc Res 52(3):221-34, 1996. PubMed: 8954864

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