產品名稱 |
Human herpesvirus 1 |
商品貨號 |
B162051 |
Classification |
Herpesviridae, Simplexvirus |
Agent |
Human herpesvirus 1 |
Strain |
ATCC-2011-1 |
Common Name |
Herpes simplex virus 1 |
Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Product Format |
frozen |
Storage Conditions |
-70°C or colder |
Images |
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Comments |
This item was isolated in cell culture at the ATCC by three passages in A-549 cells. |
Effect on Host |
CPE, clusters of rounded cells and cell death. |
Recommended Host |
A-549 (ATCC® CCL-185™) |
Growth Conditions |
Temperature: 37°C Recommendations for Infection: Plate cells 24-48 hours in advance and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus diluted to provide a MOI of 0.01. Adsorb for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute the inoculum. End adsorption by adding virus growth medium. Incubation: 1-3 days |
Name of Depositor |
ATCC |
Source |
Specimen from a clinical patient in Virginia, July 2011 |