產(chǎn)品名稱 |
68-36 |
商品貨號 |
B162185 |
Designations |
68-36 |
Species |
Mus musculus, mouse |
Depositors |
CM Disteche |
Applications |
Isolated from a flow-sorted library enriched for the derivative X(7) chromosome produced by the Cattanach translocation T(X;7)1Ct. |
Vector |
Construct size (kb): 10.69999980926514 |
Insert |
DNA: genomic Insert lengths(kb): 5.0 Gene product: DNA segment, Chr X, University of Washington-68(DNA segment, Chr X, St.Mary's Hospital-141) [DXWas68] |
Insert Size (kb) |
5.0 |
Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Shipping Information |
Distributed: freeze-dried |
Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--6.6, 4.4; HindIII--7.8, 3.2; KpnI--9.3, 1.8; PvuII--4.0, 2.7, 2.4, 1.75; BamHI--11.0. The insert contains the following restriction sites (approximate kb from the end closer to DXSmh141): PvuII--0.8, 3.5; HindIII--2.5; KpnI--3.0, 4.8. Part of a long complex repeat unit that occurs in about 50 copies. Other clones derived from this repeat are DXSmh141 and DXSmh15. Hybridizes to the X chromosome of Mus caroli. Isolated from a flow-sorted library enriched for the derivative X(7) chromosome produced by the Cattanach translocation T(X;7)1Ct. |
Classification |
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
References |
Disteche CM, et al. Comparison of the physical and recombination maps of the mouse X chromosome. Genomics 5: 177-184, 1989. PubMed: 2571569
Disteche CM, et al. Isolation of mouse X-chromosome specific DNA from an X-enriched lambda phage library derived from flow sorted chromosomes. Cytometry 2: 282-286, 1982. PubMed: 6210515
Disteche CM, et al. Isolation and characterization of two repetitive DNA fragments located near the centromere of the mouse X chromosome. Cytogenet. Cell Genet. 39: 262-268, 1985. PubMed: 2932308
Nasir J, et al. Unusual molecular characteristics of a repeat sequence island within a Giemsa-positive band on the mouse X chromosome. Proc. Natl. Acad. Sci. USA 87: 399-403, 1990. PubMed: 2296595
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