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<em>Pseudomonas aeruginosa</em> GFP
<em>Pseudomonas aeruginosa</em> GFP
規格:
貨期:
編號:B162643
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 Pseudomonas aeruginosa GFP
商品貨號 B162643
Application Fluorescence detection
Fluorescence imaging
FACS
Cell infection assays
In vivo localization
Pathogen-host interactions assays
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format frozen
Storage Conditions -80°C or colder
Preceptrol&reg; no
Type Strain no
Antibiotic Resistance Ampicillin resistance gene (bla) encoded on a plasmid. Confers resistance to 300 µg/mL ampicillin.
Comments This clone was derived from ATCC® 15692™ and contains a multicopy vector encoding the green fluorescent protein GFPmut3. This gene is expressed under the control of the Plac promoter. This construct has been designed for Gram-negative bacteria fluorescence labeling.

Vector: pUCP18-MCSgfpmut3
Vector size: 5.492kb
Vector type: plasmid
Marker: bla (ampR)
Features:
GFPmut3: fluorescent maker under the control of Plac promoter
Origin of replication: ori from pRO1600
Unique restriction sites: BamHI, EcoRI, EcoRV, SmaI, XhoI.
Double restriction sites: HindIII, PstI, SalI, XbaI.
Medium ATCC® Medium 2854: Nutrient Agar/Broth with 300 mcg/ml Ampicillin
Growth Conditions Temperature: 37°C
Atmosphere: Aerobic
Name of Depositor M Barbier
Chain of Custody Modified by ATCC (Dr. M. Barbier) <-- ATCC <-- BW Holloway <-- P Rountree
References

Barbier M and Mittar D. Novel fluorescent reporters for studying pathogen-host interactions. ASM general meeting 2014 Poster.

Choi KH, Schweizer HP. mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa. Nat. Protoc. 1(1): 153-161, 2006. PubMed: 17406227

Cox CD. Effect of pyochelin on the virulence of Pseudomonas aeruginosa. Infect. Immun. 36: 17-23, 1982. PubMed: 6804387

J. Bacteriol. 80: 362, 1960.

Holloway BW. Genetic recombination in Pseudomonas aeruginosa. J. Gen. Microbiol. 13: 572-581, 1955. PubMed: 13278508

Stanier RY, et al. The aerobic pseudomonads: a taxonomic study. J. Gen. Microbiol. 43: 159-271, 1966. PubMed: 5963505

Holloway BW, Cooper GN. Lysogenic conversion in Pseudomonas aeruginosa. J. Bacteriol. 84: 1321-1324, 1962. PubMed: 13961373

Nature 181: 855, 1958.

. . J. Pathol. Bacteriol. 80: 448, 1960.

Bradley DE. Ultrastructure of bacteriophage and bacteriocins. Bacteriol. Rev. 31: 230-314, 1967. PubMed: 4865539

Kemp MB, Hegeman GD. Genetic control of the beta-ketoadipate pathway in Pseudomonas aeruginosa. J. Bacteriol. 96: 1488-1499, 1968. PubMed: 4973125

Britigan BE, et al. Augmentation of oxidant injury to human pulmonary epithelial cells by the Pseudomonas aeruginosa siderophore pyochelin. Infect. Immun. 65: 1071-1076, 1997. PubMed: 9038317

Hovanec TA, DeLong EF. Comparative analysis of nitrifying bacteria associated with freshwater and marine aquaria. Appl. Environ. Microbiol. 62: 2888-2896, 1996. PubMed: 8702281

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