| 產(chǎn)品名稱 | Human echovirus 9 |
|---|---|
| 商品貨號 | B162821 |
| Classification | Picornaviridae, Enterovirus, Enterovirus B |
| Deposited As | Echovirus 9 |
| Agent | Human echovirus 9 |
| Strain | Vispo |
| Biosafety Level | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format | frozen 1.0 mL per vial |
| Storage Conditions | -70°C or colder |
| Images |  |
| Comments | Derived by adaptation of primary cell culture product ATCC® VR-1051™ to LLC-MK2 Derivative cells (ATCC® CCL-7.1™). ATCC® VR-1888™ and ATCC® VR-1051™ have not been compared for sequence or infectivity in primary cell culture and tissue culture. The original source material has been reported to contain Simian virus 40. |
| Effect on Host | CPE, refractile rounding and sloughing |
| Recommended Host | LLC-MK2 Derivative cells (ATCC® CCL-7.1™) |
| Growth Conditions | Temperature: 36°C Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 2 hours at 36°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Incubation: 3-4 days at 36°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80-90% of the monolayer. |
| Name of Depositor | NIH/NIAID |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
