產品名稱 |
Mumps rubulavirus |
商品貨號 |
B162829 |
Classification |
Paramyxoviridae, Rubulavirus |
Deposited As |
Mumps |
Agent |
Mumps rubulavirus |
Strain |
Torii |
Common Name |
Mumps virus |
Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Product Format |
frozen 1.0 mL per vial |
Storage Conditions |
-70°C or colder |
Images |
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Comments |
Derived by adaptation of primary cell culture product ATCC® VR-638™ to Vero cells (ATCC® CCL-81™). ATCC® VR-1880™ and ATCC® VR-638™ have not been compared for sequence or infectivity in primary cell culture and tissue culture. |
Effect on Host |
CPE, syncytia with cell degeneration and sloughing |
Recommended Host |
Vero cells (ATCC® CCL-81™) |
Growth Conditions |
Temperature: 37°C
Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g., 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g., 0.01-0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Incubation: 4-5 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
Name of Depositor |
Takeda Chem. Ind., Ltd. |
Source |
Throat swab from 5-year-old male with mumps, Japan, 1969 |