| 產(chǎn)品名稱 | Zika virus, High titer |
|---|---|
| 商品貨號 | B162841 |
| Classification | Flaviviridae, Flavivirus |
| Deposited As | Zika |
| Agent | Zika virus, High titer |
| Strain | PRVABC59 |
| Applications | Vector-borne disease research In vitro infectivity assay Antiviral studies Spiking studies ELISpot assay Virus neutralization assay Virus ultrastructural studies Immunological-based assays PCR-based assay validation Research and development Assay development Vaccine development |
| Biosafety Level | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format | frozen 0.5 mL per vial Specification: Quantitative PFUs and genome copy number reported on Certificate of Analysis; Infectious Titer: >107 TCID50/mL |
| Storage Conditions | -70°C or colder |
| Genome Sequenced Strain | Zika virus strain PRVABC59, complete genome |
| Images |  |
| Comments | ATCC® VR-1843PQ™ is a high-titer, live virus suspended in culture medium. This product was prepared from ATCC® VR-1843™ via concentration by centrifugal filtration. |
| Effect on Host | CPE, refractile rounding and sloughing |
| Recommended Host | Vero C1008 cells (ATCC® CRL-1586™) |
| Growth Conditions | Temperature: 37°C Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 60-80% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Incubation: 4-6 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor | B Russell, Centers for Disease Control and Prevention |
| Source | Human serum specimen, Puerto Rico, December 2015 |
| Year of Origin | 2015 |
| References | Dick GW, Kitchen SF, and Haddow AJ. Zika Virus. I. Isolations and Serological Specificity. Trans R Soc Trop Med Hyg 46: 509-520, 1952. PubMed: 12995440 Zhu Z, et al. Comparative genomic analysis of pre-epidemic and epidemic Zika virus strains for virological factors potentially associated with the rapidly expanding epidemic. Emerg Microbes Infect 5: e22, 2016. |
| Cross References | Nucleotide (GenBank) : KU501215 Zika virus strain PRVABC59, complete genome |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
