產品名稱 |
Gallid herpesvirus 2 |
商品貨號 |
B163016 |
Classification |
Herpesviridae, Mardivirus |
Deposited As |
Marek's disease virus type 1 |
Agent |
Gallid herpesvirus 2 |
Strain |
Md-5 |
Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Isolation |
Buffy coat cells from chicken (Md.) with lymphomas, nerve lesions |
Product Format |
frozen |
Storage Conditions |
?-70°C or colder |
Comments |
Highly cell associated. Serotype 1 Marek's disease virus. Considered a very virulent strain (vvMDV). Has high virulence in HVT immunized or genetically resistant chickens. Frozen infected cells should be thawed quickly in 37C water bath and immediately diluted in 3-5 volumes of fresh medium. After the cells attach they should be fed with fresh medium. Highly cell associated. Serotype 1 Marek's disease virus. Considered a very virulent strain (vvMDV). Has high virulence in HVT immunized or genetically resistant chickens. |
Effect on Host |
Yes, in vitro effects: Cytopathic effects in tissue culture ?CPE |
Recommended Host |
duck embryo fibroblasts chicken; chicken kidney cells; duck embryo fibroblasts |
Growth Conditions |
Temperature: 38.0°C Duration: 7 days |
Name of Depositor |
RL Witter |
Source |
Buffy coat cells from chicken (Md.) with lymphomas, nerve lesions |
References |
Witter RL. . Avian Dis. 24: 210-232, 1980.
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