產品名稱 |
Human herpesvirus 2 |
商品貨號 |
B163242 |
Classification |
Herpesviridae, Simplexvirus |
Deposited As |
Herpes simplex virus 2 |
Agent |
Human herpesvirus 2 |
Strain |
MS |
Common Name |
Herpes simplex 2 |
Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Product Format |
frozen 1 mL per vial |
Storage Conditions |
-70°C or colder |
Images |
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Comments |
Deposited as Herpes simplex virus 2. |
Effect on Host |
CPE, cell rounding, clumping, and sloughing |
Recommended Host |
Vero (ATCC® CCL-81™) |
Growth Conditions |
Temperature: 37°C Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere while rocking continuously. End adsorption by adding virus growth medium. Incubation: 2-5 days |
Name of Depositor |
WR Dowdle, K Herrmann, Centers for Disease Control and Prevention |
Special Collection |
NCRR Contract |
Source |
Brain of a 50 year old female with multiple sclerosis, Iceland, 1961 |
References |
Dowdle WR, et al. Association of antigenic type of herpesvirus hominis with site of viral recovery. J. Immunol. 99: 974-980, 1967. PubMed: 4295116
Gudnadottir M, et al. Virus isolated from the brain of a patient with multiple sclerosis. Exp. Neurol. 9: 85-95, 1964. PubMed: 14126126
Pauls FP, Dowdle WR. A serologic study of herpesvirus hominis strains by microneutralization tests. J. Immunol. 98: 941-947, 1967. PubMed: 4290495
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