產品名稱 |
Raccoonpox virus |
商品貨號 |
B163379 |
Classification |
Poxviridae, Orthopoxvirus |
Deposited As |
Raccoonpox virus, Orthopoxvirus |
Agent |
Raccoonpox virus |
Strain |
Herman |
Applications |
The virus cross-reacts serologically with the Connaught strain of vaccinia virus. Cross-reacts with Connaught strain on vaccinia virus. |
Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Isolation |
Isolated by Y.F. Herman from respiratory tract of raccoon with no clinical symptoms, Maryland, USA, 1964 |
Product Format |
frozen |
Storage Conditions |
? -70°C or colder |
Comments |
The virus does not grow well on chorioallantoic membranes after 2 to 3 passages. The virus cross-reacts serologically with the Connaught strain of vaccinia virus. Raccoons inoculated with RPV show no apparent clinical symptoms; however, their sera demonstrate strong HAI reactions. Does not grow well on CAM after 2-3 passages. Cross-reacts with Connaught strain on vaccinia virus. Raccoons inoculated with RPV show no apparent clinical symptoms; however, their sera demonstrate strong HAI reactions. |
Effect on Host |
Yes, in vitro effects: Cytopathic effects (large plaques and giant cell syncytia) in Vero cells Giant cell syncytia in Vero TC, pinpoint pocks on CAM, paralysis in sM. Yes, in vivo effects: paralysis in suckling mouse Yes, in vivo effects: pinpoint pocks on chorioallantoic membranes |
Recommended Host |
LLC-MK2 cells (ATCC CCL-7); Vero (ATCC CCL-81) Host of Choice: Vero (ATCC CCL-81) Host Range: Vero and LLC-MK2 TC, CAM, sM Vero cells (ATCC CCL-81); LLC-MK2 cells (ATCC CCL-7); CAM; suckling mouse |
Growth Conditions |
Temperature: 35.5°C Duration: 3-4 days |
Name of Depositor |
JH Nakano |
Source |
Isolated by Y.F. Herman from respiratory tract of raccoon with no clinical symptoms, Maryland, USA, 1964 |
Year of Origin |
1964 |
References |
Thomas EK, et al. Further characterization of Raccoonpox virus. Arch. Virol. 49: 217-222, 1975. PubMed: 813616
Herman YF. Bact. Proc. 64th Annual Meeting, ASM : 117, 1964.
|