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pJCZ3.1 [RW-164]
pJCZ3.1 [RW-164]
規格:
貨期:
編號:B237818
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 pJCZ3.1 [RW-164]
商品貨號 B237818
Designations pJCZ3.1 [RW-164]
Species Homo sapiens, human
Depositors RL White, Y Nakamura
Applications
RFLPs observed under normal stringency (0.1X SSC).
Vector
Construct size (kb): 8.600000381469727
Insert
DNA: genomic
Insert lengths(kb): 6.0
Gene product: DNA Segment, single copy [D19S20]
Insert Size (kb) 6.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): PstI--7.9, 0.71; EcoRI--8.6; HindIII--8.6; BamHI--4.3 doublet; HincII--7.6, 0.95; EcoRI/HindIII--6.0, 2.7; consistent with deposited DNA.
The structure of this plasmid is not consistent with the published description of pJCZ3.1. The hybridization pattern of this probe with human DNA was not checked by the ATCC.
Subcloned from cosmid JCZ3 isolated by zeta globin oligonucleotide. RFLPs observed under normal stringency (0.1X SSC).
References

Odelberg SJ, et al. Characterization of eight VNTR loci by agarose gel electrophoresis. Genomics 5: 915-924, 1989. PubMed: 2574152

Nakamura Y, et al. Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235: 1616-1622, 1987. PubMed: 3029872

Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence pJCZ3.1 on chromosome 19 [D19S20]. Nucleic Acids Res. 16: 1229, 1988. PubMed: 2894014

Peter J O'Connell, personal communication

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