| 產品名稱 | Blakeslea trispora Thaxter |
|---|---|
| 商品貨號 | B239178 |
| Strain Designations | NRRL 2456 [CBS 130.59] |
| Application | Classroom demonstration Produces beta-carotene |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format | frozen |
| Storage Conditions | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Type Strain | no |
| Preceptrol® | no |
| Genome Sequenced Strain | Yes |
| Mating Type | plus |
| Comments | Genome sequencing strain (Joint Genome Institute, Department of Energy, USA ) |
| Medium | ATCC® Medium 200: YM agar or YM broth ATCC® Medium 325: Malt extract agar (Blakeslee's formula) ATCC® Medium 340: Rabbit food agar ATCC® Medium 336: Potato dextrose agar (PDA) |
| Growth Conditions | Temperature: 24°C to 26°C Atmosphere: Typical aerobic |
| Sequenced Data |
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence GGCTCGTCCTGTTACTATCTATTTTACTGTGAACTGTTTTATTTTRCAGCGTTTGAGGGATGTTCTTGTGCTATATGGGTAGGCATAAGAAATGTTAACCGAGCTGTAATCAGGCTTAGGCTTGGTACCCTGTTTATATACTTTCAATTGATCAGATTTTAAAATGTAACATAGAGTAAAATCTATAAAACAACTTTTAACAACGGATCTCTTGGCTTTTGCATCGATGAAGAACGTAGCAAATTGCGATAACTAGTGTGAATTGCAAATTCAGTGAATCATCGAGTCTTTGAACGCATCTTGCGCTCATTGGTATTCCAGTGAGCACGCCTGTTTCAGTATCAAAAACAACCCTCATTCAAATTTTTTTTTTGAATGGTCATGAAGGAAGCTAACTTGTTGGCGACCTTTTAAATTGAGTAAGGCCTGAATTTGTTTATCTAGCCTGAACTTTTTTTTAATATAAAGGAAAGCTCTTGCGACTTGAACTTTGTTGGGGCCTCCCAAATAAAGCTTTT D1D2 region of the 28S ribosomal RNA gene CATATCAATAAGCGGAGGAAAAGAAAATAACAATGATTTCCCTAGTAACGGCGAGTGAACAGGAAAGAGCTCAAAGTTGGAACCTGTTTGGTTTAGCCAAACCGGATTGTAAACTGTAGAAGTGTTTTCCTGGCACGCCTAACGAAAAAGTCCATTGGAACATGGTGACATAGAGGGTGAGAGCCCCGTATTTTGTTAGAGCTGTTGTCTTTTGTGATACACTTTCAAAGAGTCAGGTTGTTTGGGAATGCAGCCTAAATTGGGTGGTAAATCTCACCTAAAGCTAAATATTGGCAAAAGACCGATAGCGAACAAGTACCGTGAGGGAAAGATGAAAAGAACTTTGAAAAGAGAGTTAAACAGTATGTGAAATTGTTAAAAGGGAACCGTTTGGAGCCAGACTGGCTTGATTGTAATCAATCTAGATYTCGATCTGGATGCACTTGCAGTCTATGCCTGCCAACGACAATTCATTCTGGAGGAAAAAACTAGGAGGAATGTGGCCCCTCGGGGTGTTATAGACTTTTAGAAAATACTCTGGTTTGGATTGAGGAACGCAGCAGATGCTATTTGGCGAAGGTGCTGAGTGCTTTTAGTTTTATTGGTTAGAATTTCTGCTTCGGGTGGTGCTAATCAATACGATTGGAACTCGCTTAGTTCACTTTTTATCTGCTTAGGTTGTTGGCTTAATGACTCTAAATGA |
| Name of Depositor | NRRL |
| Chain of Custody | ATCC <-- NRRL <-- G.W. Martin |
| Isolation | Soil, Panama |
| Cross References | Nucleotide (GenBank) : M36311 B.trispora 5S rRNA. |
| References | Anderson RF. Method for the production of carotenes. US Patent 2,890,989 dated Jun 16 1959 Hesseltine CW, Anderson RF. Method of making carotenes and related substances by mixed culture fermentation. US Patent 2,865,814 dated Dec 23 1958 O'Donnell KL. Zygosporangial types of several Mucoralean families In: Lower fungi in the laboratory. Athens, GA Univ., Georgia. pp. 143-144, 1978. Ciegler A, Arnold M, Anderson RF. Microbiological production of carotenoids. IV. Effect of various grains on production of beta-carotene by mated strains of Blakeslea trispora. Appl Microbiol 7: 94-101, 1959. PubMed: 13637891 Kim SW, et al. Enhanced synthesis of trisporic acid and beta-carotene production in Blakeslea trispora by addition of a non-ionic surfactant, Span 20. J. Ferment. Bioeng. 84: 330-332, 1997. Anderson RE, et al. Microbiological production of beta-carotene in shaken flasks. Agric. Food Chem. 6: 543-545, 1958. Hesseltine CW, Anderson RF. Microbiological production of carotenoids. I. Zygospores and carotene produced by intraspecific and interspecific crosses of Choanephoraceae in liquid media. Mycologia 49: 449-452, 1957. Walther G, et al. DNA barcoding in Mucorales: an inventory of biodiversity. Persoonia 30: 11-47, 2013. PubMed: 24027345 Burmester A, et al. Cleavage of beta-carotene as the first step in sexual hormone synthesis in zygomycetes is mediated by a trisporic acid regulated beta-carotene oxygenase. Fungal Genet Biol. 44: 1096-1108, 2007. PubMed: 17822929 O'Donnell K, et al. Evolutionary relationships among mucoralean fungi (Zygomycota): Evidence for family polyphyly on a large scale. Mycologia 93: 286-296, 2001. Voigt K, Wostemeyer J. Phylogeny and origin of 82 Zygomycetes from all 54 genera of the Mucorales and Mortierellales based on combined analysis of actin and translation elongation factor EF-1alpha genes. Gene 270: 113-120, 2001. PubMed: 11404008 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
