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pHAFP9.9
pHAFP9.9
規格:
貨期:
編號:B241459
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 pHAFP9.9
商品貨號 B241459
Designations pHAFP9.9
GenBank Number

M16110

Species Homo sapiens, human
Depositors A Dugaiczyk
Vector
Construct size (kb): 14.30000019073486
Insert
DNA: genomic
Insert lengths(kb): 9.899999618530273
Tissue: fetal liver
Gene product: alpha-fetoprotein( alpha-1-fetoprotein, alpha-foetoprotein, alpha-fetoglobulin) [AFP]
Alleles: B1, B2, C1, C2, D1, D2
Insert Size (kb) 9.900
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--10.0, 4.6; EcoRI--10.2, 4.4; PstI--4.3, 4.0, 2.5 (doublet), 0.9; SalI--14.0; HindIII--7.0 (doublet).
The insert contains the following restriction sites (nt position according to the published sequence): PstI--1843, 2786, 5318, 9763; HindIII--2775; BamHI--5462.
This is the EcoRI fragment extending from nt 1 to 9906 of the published sequence subcloned from lambda33. Contains exons 1-7 and an Alu repeat.
The polymorphic site is at nt 9494 of the published sequence.
References

Gibbs PE, et al. Structure, polymorphism, and novel repeated DNA elements revealed by a complete sequence of the human alpha-fetoprotein gene. Biochemistry 26: 1332-1343, 1987. PubMed: 2436661

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