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Primary Renal Mixed Epithelial Cells; Normal, Human (HREC)
Primary Renal Mixed Epithelial Cells; Normal, Human (HREC)
規格:
貨期:
編號:B244371
品牌:Mingzhoubio

標準菌株
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DNA
RNA

規格:
凍干粉
斜面
甘油
平板


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    產品名稱 Primary Renal Mixed Epithelial Cells; Normal, Human (HREC)
    商品貨號 B244371
    Organism Homo sapiens, human
    Tissue Kidney
    Cell Type Epithelial
    Morphology Cuboidal with a characteristic pattern of swirled cells; refractile
    Growth Properties Adherent
    Biosafety Level 1

    [These primary cells are not known to harbor an agent recognized to cause disease in healthy adult humans. Handle as a potentially biohazardous material under at least Biosafety Level 1 containment. Cells derived from primate lymphoid tissue may fall under the regulations of 29 CFR 1910.1030 Bloodborne Pathogens.  

    ATCC recommends that appropriate safety procedures be used when handling all primary cells and cell lines, especially those derived from human or other primate material. Detailed discussions of laboratory safety procedures are provided in Laboratory Safety: Principles and Practice, 2nd ed. (ASM Press, Washington, DC) (Fleming et al., 1995) and Caputo, J.L. Biosafety procedures in cell culture. (1988) J. Tissue Culture Methods 11:223.

    Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS Publication No. (CDC) 93-8395. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.]

    Human Material Precaution 

    All tissues used for isolation are obtained under informed consent and conform to HIPAA standards to protect the privacy of the donor’s personal health information. It is best to use caution when handling any human cells. We recommend that all human cells be accorded the same level of biosafety consideration as cells known to carry HIV. With infectious virus assays or viral antigen assays, even a negative test result may leave open the possible existence of a latent viral genome.


    Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

    Disease Normal
    Age Batch-specific
    Gender Batch-specific
    Applications
    Applications for use might include research related to hypertension; diabetes; oncology; renal fibrosis; inflammation; autoimmune disease; drug screening/development; and toxicology.
    Product Format frozen 1 mL
    Storage Conditions -130°C or below
    Comments
    A complete solution to propagate renal mixed epithelial cells with excellent morphology in low serum (0.5% FBS) conditions.
    Complete Growth Medium
    1. Obtain one Renal Epithelial Cell Growth Kit from the freezer; make sure that the caps of all components are tight.
    2. Thaw the components of the growth kit just prior to adding them to the basal medium.
    3. Obtain one bottle of Renal Epithelial Cell Basal Medium (485 mL) from cold storage.
    4. Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
    5. Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the indicated volume of each growth kit component, as indicated in Table 1, to the bottle of basal medium using a separate sterile pipette for each transfer.

    6.        Table 1. Renal Epithelial Cell Growth Kit Components 

      Component

      Subculturing

      Volume

      Volume

      Final Concentration

      Cells per Vial

      Fetal Bovine Serum (FBS)

      Sterility Tests

      2.5 mL

      Viral Testing

      0.5%

      Viability

      Triiodothyronine

      Population Doubling Capacity

      0.5 mL

      C of A

      10 nM

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