PC-3
Source: Organ: prostate
Tumor Stage: grade IV
Disease: adenocarcinoma
Derived from metastatic site: bone
DNA Profile (STR): Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 11
D5S818: 13
D7S820: 8,11
THO1: 6,7
TPOX: 8,9
vWA: 17
Comments: The PC-3 was initiated from a bone metastasis of a grade IV prostatic adenocarcinoma from a 62-year-old male Caucasian.
The cells exhibit low acid phosphatase and testosterone-5-alpha reductase activities.
DU145
Source: Organ: prostate
Disease: carcinoma
Derived from metastatic site: brain
DNA Profile (STR): D7S820: 7, 10, 11
D13S317: 12, 13, 14
D5S818: 10, 13
vWA: 17, 18, 19
THO1: 7
CSF1PO: 10, 11
TPOX: 11
D16S539: 11, 13
Amelogenin: X, Y
PC3 細(xì)胞系: 是從人前列腺癌骨轉(zhuǎn)移腫瘤中分離出來(lái)的，分化程度低，為雄激素非依賴(lài)性前列腺癌細(xì)胞，不含有內(nèi)源性的雄激素受體，具有中等強(qiáng)度的轉(zhuǎn)移潛能，被廣泛用于雄激素抵抗型的前列腺癌的研究。PC3 細(xì)胞高表達(dá) E-cadherin，不表達(dá) Vimentin
DU145 細(xì)胞: 是從前列腺癌腦轉(zhuǎn)移腫瘤中分離出來(lái)的，分化程度低，為雄激素非依賴(lài)的前列腺癌細(xì)胞，具有強(qiáng)大的轉(zhuǎn)移潛能，缺乏內(nèi)源性的雄激素受體的表達(dá)［7］。DU145 細(xì)胞完全貼壁的時(shí)間通常在 24 h 以?xún)?nèi)。具有較強(qiáng)的侵襲和促血管新生能力，高表達(dá) VEGF 和 EGFR。DU145 細(xì)胞高表達(dá) Vimentin，不表達(dá) E-cadher-in。
